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The Hyphenated system of liquid chromatography (LC) and mass spectrometry (MS) (LC/MS/MS) presents an efficient screening tool that has resolved a lot of crude plant extracts providing a quick identification of novel chemical classes in addition to the use of a small amount of source material. A key for flavonoid identification using HPLC/MS/MS analysis: is a useful and easy to use guide with uncomplicated terminology for researchers using HPLC/MS/MS in identification of their plant extracts focusing on flavonoids. This book provides an overview about the different ionization techniques in analysis of flavonoids and the necessary conditions for their separation using HPLC. It also presents clear schemes and diagrams to acquaint the reader with the reason behind the obtained fragments in their chromatograms.