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This volume constitutes the refereed proceedings of the 2nd§International Conference on Advanced Science and Technology, AST 2010,§held in Miyazaki, Japan, in June 2010.§The 16 revised full papers presented in this volume were carefully§reviewed and selected from 220 submissions. The papers address current§issues in computer science and its applications, software engineering§and its applications, bio-science and bio-technology, u- and e-service,§science and technology, database theory and application, control and§automation, signal processing, image processing and pattern recognition,§as well as grid and distributed computing.