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A new emerging field that combines nanoscale materials and biosensor technology is receiving increased attention. Nanostructures have been used to achieve direct wiring of biosensing elements to electrode surfaces, to promote bio-reactions, to impose nanobarcodes on biomaterials, and to amplify the signal from bio-recognition events. Nanomaterials based biosensors have found wide spread applications in the environmental and medical applications for their sensitivity, specificity, rapidity, simplicity, and cost-effectiveness. In the same pursuit, Biosensors Nanotechnology provides detailed review chapters on a range of nanostructures such as nanoparticles, nanowires, nanotubes, nanoribbons, nanorods, nanobelts and nanosheets in the construction of biosensors with set applications of biosensors nanotechnology for biological and chemical analyses, food safety industry, biomedical diagnostics, clinical detection, and environmental monitoring. The senior contributors write on the following topics: ZnO and graphene microelectrode applications in biosensingAssembly of polymers/metal nanoparticlesGold nanoparticle-based electrochemical biosensorsImpedimetric DNA sensing employing nanomaterialsGraphene and carbon nanotube based biosensorsThe-state-of-art of nanomedicineComputational nanochemistry studyBFPF green fluorescent protein chromophoreBiosynthesis of metal nanoparticlesIonic discotic liquid crystalsRole of advanced materials as nanosensors in water treatmentBioconjugated-nanoporous gold films in electrochemical biosensorsCombination of molecular imprinting and nanotechnologyRecent development of fiber reinforced composite materialsPrincipal and properties of multiferroics and ceramics