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This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems. * Provides researchers with solutions in lab environments* Features an array of essential methods, including endocytic pathways, membranes, mitochondria, and in vitro motility* Information on a plethora of technologies needed to tackle complex problems