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Contraception: An Herbal Medicine Approach of Citrus medica Seeds for Fertility Regulation, this research book focused on the evaluation of contraceptive activities of C. medica seeds on albino rats. Out of the tested three crude extracts, petroleum ether extract found more potential on fertility regulation, judged by phytochemical analysis, acute and chronic toxicity studies, antiimplantation, abortifacient, antiovulatory, estrogenic, progestogenic activities. Results were analysed by gravimetric, histological, histometrical, biochemical, antioxidant assays, hormonal profile by RIA and withdrawal studies with applying statistical analysis Student's 't' Test and also its TLC fractions were tested and found potential at the dose level of 50 and 100mg/kg/b.w. of fraction II in significantly. Spectroscopic analysis like UV, GCMS, IR and H1 NMR were resulted isolated compound is fatty acid mixture of fraction II, structure not elucidated and characterized due to the nature of fatty acid moiety. Hence, the fraction II is rich in fatty acid and shown contraceptive effects without obtrusive side effects in rats and might be use it for contraceptive purpose.