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This timely new volume in the highly acclaimed Neuromethods series focuses on major new advances in understanding the signaling mechanisms employed by neural tissues.§Chapters, contributed by experts in the field, provide thorough, up-to-date coverage of a variety of topics and techniques, including: single-cell imaging technology phosphorylation of target proteins analysis of phosphoinositides and inositol phosphates inositol trisphosphate and intracellular calcium guanine nucleotide-binding proteins ion channel gating measurement of intracellular calcium with fluorescent calcium indicators protein kinase C synthetic analogs of intracellular messengers cyclic nucleotides caged intracellular messengers intracellular regulators and components of the exocytotic pathway intracellular messengers in vertebrate and invertebrate photoreceptors.§Neuromethods 20 Intracellular Messengers is an essential resource for neurobiologiests at all levels of research.