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Petri Net Theory is a mathematical formalism to describe the behaviour of systems with concurrent processes, and has been applied mainly in technical applications such as in computer scPetri Net Theory is a mathematical formalism to describe the behaviour of systems with concurrent processes, and has been applied mainly in technical applications such as in computer science for modelling distributed systems.§This reader-friendly text covers the important theme of Petri net-based analysis of complex systems, explaining and summarizing recent applications of Petri nets in systems biology for biochemical network modeling. Readers will gain in-depth understanding into this widely used technique and will be enabled to apply the different Petri net-based techniques to model biochemical systems. Furthermore it offers a good foundation in biology and Petri net theory, and an overview of existing tools.§This comprehensive text is an essential resource and guide to the topic of Petri net-based analysis of complex systems and be valuable to both practitioners and researchers in the topic, as well as graduates wishing to learn more about the field.