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This book covers aspects of biological nitrogen fixation along with the unique signaling and interaction between the diazotrophic bacteria and plants, especially the non-legumes. Nitrogen is the most important growth-limiting nutrient in the ecosystems and biological nitrogen fixation involving microbial symbionts, mainly rhizobia and legumes holds enormous interest across the globe. However, free-living rhizobacteria of non-legumes especially cereals, also establish themselves within the root system, fixing nitrogen and contributing to plant productivity, soil fertility, and agricultural sustainability. These non-symbiotic nitrogen fixers additionally exhibit various plant growth-promoting traits elevating productivity, fortifying nutrient content, and managing water stress in plants. The recent perspectives highlighting the mechanisms and background of non-symbiotic nitrogen fixation provide answers to unravel the potential of nitrogenase and various spectra of habitats of rhizobia and other diazotrophic bacteria. Further, the application of genetic engineering and the development of nitrogen-fixing cereals can provide a possible solution to the problem of food shortage. The book includes various scientific inputs providing comprehensive knowledge about the emergence of agricultural sustainability through nitrogen-fixing bacteria. The book illustrates the systematic mechanisms involved in biological nitrogen fixation through various illustrations, schematic drawings, and flow charts aiding in better understanding. The chapters elaborate on the physiology and metabolism of plant-bacteria interaction in different crops under diverse environmental conditions. Thus, the volume will provide a holistic scenario helping in advancing the novel plant-microbe interactions, cell-signaling, and plant-molecular interactions. The book will assist the agronomists, microbiologists, ecologists, plant pathologists, molecular biologists, environmentalists, policymakers, conservationists, and NGOs to develop biofertilizers and bioinoculants using various genera of microbes and contribute to the targets of sustainable goals in an eco-friendly manner.