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This book presents the initial development of oligo§(ethylene glycol) (OEG) based nonfouling -protein §and cell resistant- coatings that can be applied to §a wide range of biomedical applications. A §generalized method for creating functionalized §nonfouling surfaces was developed by §conducting Surface-Initiated Atom Transfer Radical §Polymerization of OEG methyl §methacrylate (SI-ATRP of OEGMA) from gold (metallic §materials), glass and silicon oxide. Thickness-§density profile of poly(OEGMA) was constructed based §on SI-ATRP from mixed self-assembled monolayers on §gold. For the first time, we constructed a map of §protein resistance of PEG coated surfaces, which §reveals the relationship between the poly(OEGMA) §coatings and their protein adsorption. Applications §of this technology were demonstrated on the §fabrication of protein microarray, micro/nano-§patterns for in vitro cell culturing and improved §biocompatibility for in vivo implantation. This work §provided a sound foundation for future work focused §on the optimization of material-specific coating §conditions and in vivo biocompatibility study of OEG §coated biomedical devices.