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Gingival and Periodontal diseases represent a major concern both in dentistry and medicine. The majority of the contributing factors and causes in the etiology of these diseases are reduced or treated with ozone in all its application forms (gas, water, oil). The beneficial biological effects of ozone, its anti-microbial activity, oxidation of bio-molecules precursors and microbial toxins implicated in periodontal diseases and its healing and tissue regeneration properties, make the use of ozone well indicated in all stages of gingival and periodontal diseases. The purpose is to provide a general review about the clinical applications of ozone in periodontics and to summarize the in vitro and in vivo studies in Periodontics in which ozone has been used. This would be of importance to future researchers in terms of what has been tried and what the potentials are for the clinical application of ozone in Periodontics.