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The book is considered as a survey of some secondary metabolites present in Viola odorata L., collected from plant nursery in Baghdad between April and July, followed by the fractionation and isolation of Stigmasterol and ß-sitosterol.The biological study demonstrated the effect of the Stigmasterol and ß-sitosterol found in Viola odorata L. as antioxidant and antibacterial.... Extraction of Stigmasterol and ß-sitosterol as a crude extract from each part of the plant roots, leaves, flowers and seeds using (Petroleum ether and Methanol) to a qualitative and quantitative estimation of Stigmasterol and ß-sitosterol in each part of the plant by using TLC, HPLC and CC.