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This work aimed to evaluate the fungicidal activity of oil-in-water (O/W) nanoemulsions of hydroalcoholic extract andessential oil of sprouts of Syzygium aromaticum. The action of the biotechnological product was tested against three strains of fungi Aspergillus niger, Colletotrichum gloeosporioides, Penicillium chrysogenum. For antifungal action of the essential oil nanoemulsion, the MIC obtained was 25µg mL-1,25 µg mL-1and 50µgmL-1 against A. niger, C. gloeosporioides and P. chrysogenum, respectively, for the hydroalcoholic extract nanoemulsion the result was 50 µg mL-1, 50 µg mL-1and 100 µg mL-1 for A. niger, C. gloeosporioides and P. chrysogenum, respectively. On the other hand, the CIM dthe nanoemulsion of the essential oil showed activity against A. niger of 25 µg mL-1 and against C. gloeosporioides it showed inhibition in 25 µg mL-1 and in the nanoemulsion of the hydroalcoholic extract it had its best performance in front of A. niger of 50 µg mL-1and in front of C. gloeosporioides, which showed inhibition in 50µg mL-1. Finally, this study points to the nanoemulsion of S. aromaticum as an alternative fungicide in the control and combat of pathogenic fungi.